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. 2023 Sep 12;12(1):2240670. doi: 10.1080/2162402X.2023.2240670

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© 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 1. — Schematic Overview of the Study. There are four key stages: I. HPC Derivation and Characterization; II. Organoid System Engraftment and HPC Mouse Transplantation; III. NK Characterization; IV. NK Functional Assays. HPCs are hematopoietic progenitor cells that were analyzed at 3 differentiation time points (HPC1, HPC2, HPC3). HSCG is Hematopoietic Stem Cell Growth Medium. K562, Jurkat, DIPG, and GBM are chronic myelogenous leukemia, acute T-lymphoblastic leukemia, diffuse intrinsic pontine glioma, and glioblastoma multiforme cell lines, respectively.