Fig 7.

Macrophages from young APOE mice have diminished inflammatory responses to L. monocytogenes infection compared to macrophages derived from aged mice. (A) Macrophages cultured from the bone marrow of young mice (males and females) were infected with L. monocytogenes at MOI = 0.1 for 5 hpi and then culture supernatants were collected for multiplex immunoassay. Only cytokines and chemokines that were upregulated following L. monocytogenes infection are shown. Symbols represent individual mice; bars indicate median values for data pooled from multiple experiments. Two-way ANOVA was performed. (B and C) Principal component analysis was performed using selected cytokine and chemokine immunoassay data (young data are shown in panel A; aged data are shown in Fig. 3) that were upregulated in young or old macrophage culture supernatant 5 hpi (B) or 24 hpi in splenocyte culture supernatants (C). Symbols are biological replicates (samples collected from cells isolated from individual mice).