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. 2023 Aug 31;14:1230723. doi: 10.3389/fpls.2023.1230723

Figure 4.

Figure 4

Overexpression of PRK. (A) Design of the transgene driving PRK expression and the genetic background (ΔPRK or CC-4533) for the A2, β3 and D5 strains (B) Anti-PRK western blot on total soluble protein extracts of the ΔPRK, CC-4533, and transformed strains as indicated. 100% corresponds to 12 μg of total protein. A gradient of the total protein extract of the strain CC-4533 was used for the quantification of the PRK content in the different complemented strains, indicated as PRK level (%). (C-E) Growth curves of CC-4533 and transformed strains. Cultures were inoculated at 105 cells/mL and incubated in HSM at 25°C under three distinct light intensities as indicated.