Extended data Fig. 6 |. Identification of the ions in the NIS-ReO4− structure.

a. The ions transported by NIS were identified by evaluating the map density along 24 lines passing through each site (circled in yellow on images of map slices). Values are plotted for each line and the spherically-averaged mean is plotted in black (lower panels). b. Effects of substitutions in binding-site residues on ReO₄⁻ transport at steady state (measured at 3 μM ReO₄⁻ and 140 mM Na⁺ for 30 min (values obtained in the presence of ClO₄⁻, which are < 10% of the values obtained in its absence, have already been subtracted). Values are normalized to those obtained with WT NIS. Values represent averages of the results from two or three different experiments, each of which was carried out in triplicate (n ≥ 6) and reported as pmol ReO₄⁻ accumulated/μg DNA ± s.e.m.