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. 2023 Apr 19;38(6):1047–1059. doi: 10.1093/humrep/dead069

Figure 3.

Figure 3.

Specific reduction in endometrial uterine natural killer 3 (uNK3) cells after hormonal stimulation. (A) Raw flow cytometry gating strategy to identify uterine natural killer 1 (uNK1) (CD39+CD103−), uNK2 (CD39−CD103−), and uNK3 (CD39−CD103+) cells in the endometrium (‘endo’). Events shown are live, singlet, and CD45+CD3−CD56+ NK cells. (B) uNK1, uNK2, and uNK3 cells found in endometrium phenocopy previously described decidual NK1, NK2, and NK3 cells based on expression of KIR2DL1, Perforin, and CD18 (integrin beta 2 (ITGB2)). (C) Reduction in density of endometrial NK3 cells after hormonal stimulation. Each point represents a single tissue sample and bar represents the mean of each set of samples. Naturally cycling subjects (n = 11), hormonally stimulated subjects (n = 5). *P = 0.025 by unpaired t-test.