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. 2023 Mar 21;13(9):3930–3944. doi: 10.1016/j.apsb.2023.03.017

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© 2023 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Effect of compound 3 on IL-17A produced from T cells under Th17-priming conditions. Splenic cells were grown in an environment conducive to Th17-priming for three days with different concentrations of compounds. (A and B) Analysis of IL-17A inhibition ratios in CD4⁺ T cells utilizing flow cytometry of compounds 3 and 11. (C–H) Flow cytometry analysis of cell frequency and counts of IL-17A-GFP⁺ gated CD4⁺ or CD8⁺T cells. (I–L) Quantitative analysis of cell apoptosis by 7-AAD and annexin V-APC staining. (M) ELISA was implemented to measure the levels of IL-17A in the cell culture supernatant. (N) Expression of IL-17A and RORγt assessed by Western blotting. Data were reported as mean ± SD for n = 3. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. compound 3 (0 μmol/L) group.