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. 2023 Jun 1;13(9):3782–3801. doi: 10.1016/j.apsb.2023.05.034

Figure 2.

Figure 2

Comparison between Taspine and SMU-Y6. (A) SEAP signaling of Taspine and SMU-Y6 in HEK-Blue hTLR2 cells. HEK-Blue hTLR2 cells were treated with Pam3CSK4 100 ng/mL and Taspine or SMU-Y6 for 24 h. The supernatant was collected for SEAP signaling. (B) Comparison of water solubility between Taspine and SMU-Y6. (C) TNF-α or IL-6 in supernatants of primary murine peritoneal macrophage cells after treatment with indicated Pam3CSK4 (100 ng/mL) and differing concentrations of SMU-Y6 for 24 h. TNF-α in supernatants of THP-1 cells after treatment with Pam3CSK4 (100 ng/mL) and differing concentrations of SMU-Y6 for 24 h. (D) TNF-α or IL-6 in supernatants of primary murine peritoneal macrophage cells after treatment with indicated LPS (100 ng/mL) and differing concentrations of SMU-Y6 for 24 h. TNF-α in supernatants of THP-1 cells after treatment with indicated LPS (100 ng/mL) and differing concentrations of SMU-Y6 for 24 h. (E) IL-6 in supernatants of primary murine peritoneal macrophage cells after treatment with indicated Poly I:C (10 μg/mL), R848 (10 μg/mL), ODN 2395 (5 μmol/L) and differing concentrations of SMU-Y6 for 24 h. (F) IL-6 in supernatants of primary murine peritoneal macrophage cells (TLR2−/−) after treatment with indicated Poly I:C (10 μg/mL), R848 (10 μg/mL), ODN 2395 (5 μmol/mL) and differing concentrations of SMU-Y6 for 24 h. Data presented is mean ± SD and the figures shown are representative of three independent experiments.