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. 2023 Jun 23;30(9):1285–1295. doi: 10.1038/s41417-023-00637-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a IGF2BP3 levels in supernatants from EWS cells evaluated by ELISA assay. The bars represent the mean ± SE of two independent biological experiments with at least two replicates/each. b on the left, a schematic of the experimental plan to obtain EVs and EVs-depleted supernatant. The figure was created with BioRender.com; on the right, western blotting showing IGF2BP3 expression in complete supernatant (Pre-EQ S), EVs-depleted supernatant (Post-EQ S) and EVs obtained from A673, TC-71 and EW#5-C cells. Representative western blots of at least two independent experiments are shown. c Western blots depicting the expression of IGF2BP3 and EVs markers (TSG101 and ALIX) are shown. Protein analysis was performed on lysates from IGF2BP3-depleted (#18, #54, #46, #73, sg-IGF2BP3-1, sg-IGF2BP3-2), parental or mock-silenced (shNC and sgNC) cells and related EVs. CALN was used as a control to confirm that EVs were not contaminated with cellular material.