Fig. 3. IGF2BP3 is associated with a specific miRNA signature in EVs capable to clusterize EWS patients with high or low expression of IGF2BP3 at the tissue level.

a Heatmap of differentially expressed miRNAs in IGF2BP3pos EVs versus IGF2BP3neg EVs extracted from A673 or TC-71 experimental models, respectively. b Heatmap unsupervised hierarchical clustering analysis of the commonly differentially expressed miRNA between IGF2BP3-EV-associated signature and those that were present in the dataset ArrayExpress accession: E-TABM-1100. The hierarchical clustering algorithm showed a distinct cluster of EWS patients with high expression of IGF2BP3 in the tumors compared to those with low expression. In the matrix, each row represents a miRNA, and each column represents a sample. The color scale illustrates the relative expression levels (z-score) of miRNAs across all samples: red represents the expression level above the mean and blue represents the expression lower than the mean.