Figure 7.

Absence of ADNP at the Il13 promoter reduces local H3K27 acetylation and DNA accessibility

(A) Representative ATAC-seq and H3K27ac ChIP-seq tracks from Th2 cells from Cd4^Cre or Cd4^creAdnp^fl/fl mice in the type 2 cytokine locus. Data are representative of 2 biological replicates.

(B) Detection of p300 protein (218 kDa) in immunocomplex generated with Th2 cell nuclear lysate co-immunoprecipitated with anti-ADNP antibody. INP, input nuclear extract; SN, supernatant; IP, immunoprecipitation elution. Data are representative of 2 independent experiments. IB refers to immunoblotting antibody.

(C) Average ChIP-seq signal over all H3K27ac peaks or over ADNP-CHD4-BRG1 peaks in Cd4^Cre or Cd4^CreAdnp^fl/fl Th2 cells. Data are representative of 3 biological replicates.

(D) Average ATAC-seq signal over all H3K27ac peaks or over H3K27ac peaks that were reduced in Cd4^CreAdnp^fl/fl Th2 cells. Data are representative of 2 biological replicates.

(E) KEGG pathway analysis of genes associated to the genomic regions where H23K27ac was reduced in Cd4^CreAdnp^fl/fl Th2 cells. All shown pathways were enriched (p < 0.05).

(F) Representative binding profiles of ADNP, CHD4, BRG1, and H3K27ac in Th2 cells of Cd4^Cre or Cd4^CreAdnp^fl/fl mice at the Maf and Il4ra loci. Data are representative of 2 biological replicates.

See also Figure S7 and Table S6.