. 2023 Aug 17;83(16):2911–2924.e16. doi: 10.1016/j.molcel.2023.06.035

Table 1.

Cryo-EM statistics

	Budding yeast	Budding yeast	Budding yeast	Human	Human
	replisome:Pol α-primase	replisome:Pol α-primase	replisome:Pol α-primase	replisome:Pol α-primase	replisome:Pol α-primase
	60 nt 5′ flap (−) Ctf4	60 nt 5′ flap (+) Ctf4. CIP #1	60 nt 5′ flap (+) Ctf4. CIP #2	60 nt 5′ flap	15 nt 5′ flap
	(EMDB-16322)	(EMDB-15902)	(EMDB-15902)	(EMDB-15341)	(EMD-15922)
	(PDB: 8B9C)	(PDB: 8B9A)	(PDB: 8B9B)	(PDB: 8B9D)	–

Data collection and processing

Magnification	81,000 ×	81,000×	81,000×	81,000×	81,000×
Voltage (kV)	300	300	300	300	300
Electron exposure (e⁻/Å²)	40.184	40.184	40.184	37.8	88.5
Defocus range (μm)	1.5–3	1.5–3	1.5–3	1.5–3	3.5–0.9
Pixel size (Å) (super resoution)	0.86	0.86	0.86	1.23	1.07
Symetry imposed	none	none	none	none	none
Movies collected	12,819	12,819	12,819	7,355	6,718
Initial particle images (no.)	2,003,322	2,003,322	2,003,322	1,535,548	724,557
Final particle images (no.)	100,179	54,970	44,970	174,696	258,339
Map resolution (Å) (0.143 FSC threshold)	3.34	3.5	3.5	3.4	3.3
Map resolution range (Å)	2.8–12	2.9–12	2.9–12	2.8–12	3.0–12

Refinement

Initial model used (PDB code)	PDB: 6SKL	PDB: 6SKL	PDB: 6SKL	PDB: 7PFO	–
Model resolution (Å) (0.5 FSC threshold)	4.1	4.2	4.2	4.1	–
Map sharpening B factor (Å²)	−20 to −50	−20 to −50	−20 to −50	−20 to −50	–

Model composition

Non-hydrogen atoms	59,119	69,886	69,866	66,207	–
Protein residues	7,193	8,528	8,528	8,108	–
Ligands	4 AMP-PNP	4 AMP-PNP	4 AMP-PNP	3 AMP-PNP	–
	4 Mg²⁺, 4 Zn²⁺	4 Mg²⁺, 4 Zn²⁺	4 Mg²⁺, 4 Zn²⁺	3 Mg²⁺, 4 Zn²⁺	–

RMSDs

Bond lengths (Å)	0.023	0.011	0.01	0.027	–
Bond angles (°)	1.913	1.396	1.101	2.66	–

Validation

MolProbity score	0.73	0.75	0.77	0.78	–
Clashscore	0.32	0.62	0.69	0.32	–
Poor rotamers (%)	0.29	0.5	0.53	0.66	–

Ramachandran plot

Favored (%)	97.42	97.81	97.81	97.12	–
Allowed (%)	2.58	2.19	2.19	2.88	–
Disallowed (%)	0	0	0	0	–