Figure S2.

Identification of candidate quality control factors for unassembled CCT subunits, related to Figure 1

(A) CCT subunits with a C-terminal TST were translated in RRL, affinity-purified under native conditions via the TST, and analyzed by label-free quantitative mass spectrometry. The volcano plots illustrate proteins enriched in CCT subunit pull-downs compared with control pull-downs (see method details for details). The log₂ fold difference (x axis) is plotted against the −log₁₀ adjusted p value (y axis). The statistical analysis was done using a two-sided Student’s t test with Benjamini-Hochberg correction for multiple comparisons. The bait and non-bait CCT subunits are shown as magenta and yellow dots, respectively. E3 ligases, including components of E3 ligase complexes, are in blue. ZNRD2 (also called SSSCA1) is in green.

(B) CCT subunits with a C-terminal TST were translated in RRL, affinity-purified under native conditions, and analyzed by immunoblotting for HECTD3, HERC1, HERC2, and ZNRD2. The CCT subunit was detected via the TST. Two amounts of total RRL were analyzed in parallel.