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. 2023 Sep 14;6:942. doi: 10.1038/s42003-023-05330-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Spegβ and Spegα expression in the TA of HA-Speg mice (homozygous) compared to controls (WT) (n = 7). A representative western blot is shown on the left. Total protein (in all panels) was obtained using stain-free gels (Bio-Rad), and after transfer of the proteins to the PVDF membrane (Immobilon^®-FL, Millipore), the PVDF membrane was imaged by using a ChemiDoc^TM MP imaging system (Bio-Rad). b Spegβ and Spegα expression in the TA of Speg-KO mice (homozygous) compared to controls (Speg^fl/fl) (n = 4 each). A representative western blot is shown on the left. c Spegβ and Spegα expression in hearts of HA-Speg mice compared to control mice (n = 10 each). A representative western blot is shown on the left. d Spegβ and Spegα expression in the hearts of Speg-KO mice (homozygous) compared to controls (Speg^fl/fl) (n = 4 each). A representative western blot is shown on the left. e Expression of mRNA for Spegα, β, Apeg-1 + Bpeg in TA muscles of control (n = 4–5) and HA-tagged Speg mice (n = 4–5), respectively. P values are indicated as analyzed by Welch’s t test. All statistical tests are two-sided. Data are represented as mean ± standard deviation. f Expression of mRNA for Spegβ, Spegα, Apeg-1, and Bpeg in hearts of control (n = 4–5) and HA-tagged Speg mice (n = 4–5), respectively. P values are indicated as analyzed by Welch’s t test. All statistical tests are two-sided. g Immunoprecipitation with HA antibody of homogenate from HA-Speg and control mice. The figure shows western blot of total proteins and western bot with Speg antibody and HA antibody. h ECC protein levels in the TA of Speg-KO mice compared to controls with a representative western blot (n = 7 each). i ECC protein levels in TA of Speg-KO mice compared to controls with a representative western blot (n = 4 each). j ECC protein levels in the hearts of Speg-KO mice compared to controls with a representative western blot (n = 6 each). k ECC protein levels in hearts of Speg-KO mice compared to controls with a representative western blot (n = 6 each). All mice used for the data in this figure were male. Data are shown as the mean ± SD. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.