FIG. 2.

Construction of a standard curve for quantitation of HSV-1. (A) Agarose-ethidium bromide gel; (B) image analysis of the gel in panel A; (C) standard curve plotted from the data in the table at the bottom. In the gels, the first band is 250 copies of the 123-bp IS. The next six lanes contain 100, 200, 500, 1,000, 2,000, and 5,000 copies of HSV DNA, respectively, plus 250 copies of IS. The last lane in the agarose gel contains a molecular size marker (1,000, 700, 500, 400, 300, 200, 100, and 50 bp). The table shows the pixel intensities of the amount of HSV/amount of IS. The double bands are the 148-bp product of HSV DNA and the 123-bp product of the IS and are seen in the lanes containing 100, 200, 500, and 1,000 copies of HSV DNA.