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. 2023 Sep 14;14:5690. doi: 10.1038/s41467-023-41302-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic of WNT agonism (CHIR) or WNT antagonism (XAV939) or no WNT manipulation (base media) at different timepoints during PGCLC monolayer differentiation. b FACS data showing efficiency of generating NANOG+ PGCLCs under different conditions of WNT pathway manipulation at Day 2.5 and Day 3.5. Data are presented as mean values ± SEM. Statistical test–two-way ANOVA, with Tukey multiple testing correction. n = 3 biological replicates/group for all except for CHIR99021 where n = 4 biological replicates/group. Adjusted P values are shown above error bars. Source data are provided as a Source Data file. c Log2 normalized expression levels of PGC and mesodermal markers in Base media vs. XAV939 vs. CHIR treated samples at different timepoints. n = 3 biological replicates/group.