Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 14;14:5690. doi: 10.1038/s41467-023-41302-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Schematic of the 2D monolayer PGCLC differentiation protocol reported in this manuscript. b Flow cytometry analysis of NANOS3-mCherry hESC shows fluorescent reporter expression before or after 3.5 days of differentiation. c qPCR analysis of NANOS3-mCherry+ PGCLCs and NANOS3-mCherry- non-PGCLCs derived after 3.5 days of differentiation, as shown in h; as a negative control, undifferentiated hPSCs (D0) were also analyzed, and gene expression is shown relative to undifferentiated hPSCs (which was set = 1.0). Data are presented as mean values. Source data are provided as a Source Data file. d Immunostaining of hPSCs differentiated for D3.5 showing expression of PGC markers in a subset of cells (nuclear counterstain: DAPI). Scale bar = 100 μm. e Validation of protein expression in D3.5 human ESCs. Each panel indicates the corresponding marker. The graph on the right represents the quantification of triple positive cells at D3.5 in the differentiation protocol. See Materials and Methods for details on quantification method. Each column represents mean with SEM for at least two biological replicates. n = 19,687 for H1, n = 57,067 for H9. DAPI was used as nuclear counterstain. Representative of two independent experiments. P values are shown above bars; error bars = standard error of mean. Statistical test–unpaired t test with Welch’s correction. Source data are provided as a Source Data file.