FIGURE 1.

Schematic diagram of RAA implementation for real-time detection. This figure was drawn by Figdraw. The single fluorogenic probe includes fluorophor, quencher and 3′block, and exonuclease cannot recognize the single fluorogenic probe. As the probe binds to the target DNA, exonuclease recognizes tetrahydrofuran on the probe abasic site and cleavage them, allowing the fluorophor and quencher to separate and fluoresce. At the same time, DNA polymerase unblocks the 3′block and completes the chain extension.