TABLE 2.

Comparison of clinical applications between RT-RAA and RT-qPCR.

Compare items	RT-RAA	RT-qPCR	References
Principle differences	SSB keeps the template in a single-stranded notch state and nucleic acid amplification is uninterrupted at 37°C.	Reamplification required chain denaturation at 94°C.	Zhang et al. (2017)
Reaction process	In the whole reaction process, RNA can be directly used as a template to be added to the amplification system, and reverse transcription and amplification are carried out simultaneously.	For RNA amplification, it is necessary to perform reverse transcription reaction first, which is to reverse transcribe RNA into cDNA, and then use cDNA as template for nucleic acid amplification.	Li et al. (2022)
Reaction temperature	The reaction can be carried out at a constant temperature ranging from 37°C to 42°C.	Three temperatures are required for continuous circulation, such as 95°C, 72°C, 55°C, and the temperature must be precisely controlled.	Shen et al. (2019)
Reaction time	20 min.	At least 120 min.	Chen et al. (2020)
Detection equipment	Small fluorescent detector.	RT-qPCR instrument.	Yao et al. (2022)
Detection site	On site.	PCR special laboratory.	Tang et al. (2021)
Detection personnel	On-site personnel.	Medical professional.	Lee et al. (2020b)