Table 1.
Quantification of organoids derived in different culture environments. Data are shown as mean ± SD.
| Culture Medium | Treatment | Percentage (%) |
|---|---|---|
| WENRAS (control media) |
LFD | 100 |
| WENRAS + Nutlin (only p53 mutant survive) |
LFD LFD + Red 40 |
20 ± 2.3 52 ± 5.1* |
| ENRAS (only APC mutant survive) |
LFD LFD + Red 40 |
1 ± 0.4 1 ± 0.5 |
| WENRAS (control media) |
HFD | 100 |
| WENRAS + Nutlin (only p53 mutant survive) |
HFD HFD + Red 40 |
23 ± 2.5 46 ± 4.8** |
| ENRAS (only APC mutant survive) |
HFD HFD + Red 40 |
16 ± 1.8 20 ± 2.2 |
* indicates a significant difference from LFD/WENRAS+Nutlin
** indicates a significant difference from HFD/WENRAS+Nutlin