Figure 4.

Angptl2 expression during mouse palate and limb development

(A–E) Mouse embryos at gestational day 13 (GD13) or GD14.5 were stained by in situ hybridization to visualize Angptl2 expression. Whole-mount tissues were imaged to view the developing palatal shelves and upper lip (A and B). Subsequent coronal sections illustrate prominent staining in mesenchymal tissue of the palatal shelves (A′ and B′). qPCR was conducted on mesenchyme isolated from maxillary process/palatal shelf tissue from mice at indicated time points (C). Each value represents the mean ± SEM of n = 3 samples isolated from individual embryos. Forelimbs from GD13 and 14.5 mouse embryos were also stained by in situ hybridization to visualize Angptl2 expression (D and E). A section through the first digit illustrates staining restricted to the mesenchyme adjacent to apical ectodermal ridge. Similar domains of expression were observed in hindlimbs. Scale bars, 0.50 mm.