| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Chicken polyclonal anti-POU3F4 | Coate et al., 2012,16 Aves Labs | RRID: AB_2814704 |
| Rat monoclonal anti-CD326 (Ep-CAM) | Biolegend | Cat# 118213, RRID:AB_1134105 |
| Rabbit polyclonal anti-MYO6 | Proteus Biosciences | Cat# 25–6791, RRID:AB_10013626 |
| Mouse monoclonal anti-KCNQ1 | Santa Cruz Biotechnology | Cat# sc-365186, RRID:AB_10707688 |
| Mouse polyclonal anti-TUJ1 | Biolegend | Cat# MMS-435P, RRID: AB_2313773 |
| Rabbit polyclonal anti-EMILIN2 | Amma et al., 200322 | RRID:AB_2941943 |
| Rabbit monoclonal anti-TGFBI | Abcam | Cat# ab170874, RRID:AB_2895231 |
| Rabbit monoclonal anti-RUNX2 | Cell Signaling Technology | Cat# 12556, RRID:AB_2732805 |
| Rabbit polyclonal anti-CAR3 | Proteintech | Cat# 15197-1-AP, RRID:AB_2243603 |
| Critical commercial assays | ||
| Chromium single cell 3′reagent kit V3 | 10x Genomics | Cat# PN-1000075 |
| Deposited data | ||
| E15 and P7 raw cochlear scRNA-seq datasets | This paper | GEO: GSE217727 |
| E15 and P7 analyzed cochlear scRNA-seq datasets | This paper | UMgEAR.org/mesenchyme |
| E16 and P7 cochlear epithelial scRNA-seq datasets | Kolla et al., 20201 | GEO: GSE137299 |
| Experimental models: Organisms/strains | ||
| Mouse: Crl:CD1(ICR) | Charles River | RRID:IMSR_CRL:022 |
| Mouse: C57BL/6J Pou3f4tm1Cren/y | Phippard et al., 199914 | RRID:MGI:3046172 |
| Oligonucleotides | ||
| Primer: Pou3f4 Forward Primer: CACTCTGATGAA GAGACTCCAAC | This paper | N/A |
| Primer: Pou3f4 Reverse Primer: CACCGTGTGCGA ATAAACCTC | This paper | N/A |
| Software and algorithms | ||
| ImageJ (FIJI) | Schindelin et al., 2012111 | RRID:SCR_002285 |
| R Project for Statistical Computing | http://www.r-project.org/ | RRID:SCR_001905 |
| Cell Ranger | 10x genomics112,113 | RRID:SCR_017344 |
| Seurat (R package) | https://satijalab.org/seurat/114,115 | RRID:SCR_016341 |
| IpathwayGuide | Advaita Bio116 | N/A |
| RcisTarget (R package) | Aibar et al., 201733 | N/A |
| EnhancedVolcano (R package) | https://bioconductor.org/packages/EnhancedVolcano | RRID:SCR_018931 |
| Monocle3 (R package) | https://cole-trapnell-lab.github.io/monocle3117,118,119 | RRID:SCR_018685 |
| gene Expression Analysis Resource (gEAR) | https://umgear.org31 | RRID:SCR_017467 |
| Biorender | http://biorender.com | RRID:SCR_018361 |
| CellChat (R package) | https://github.com/sqjin/CellChat54 | RRID:SCR_021946 |
| Other | ||
| Chromium Controller | 10x Genomics | N/A |
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