| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit Polyclonal Tubulin β 3 (TUJ1) (1:500) | Abcam | Cat# ab18207, RRID: AB_444319 |
| Chicken Polyclonal Glial Fibrillary Acidic Protein (GFAP) (1:2000) | Abcam | Cat# ab4674, RRID: AB_304558 |
| Goat Polyclonal Synaptophysin (Syn) (1:2000) | RD | Cat# AF5555, RRID: AB_2198864 |
| Rabbit polyclonal postsynaptic density protein 95 (PSD-95) (1:1000) | Thermo Fisher | Cat# 51-6900, RRID: AB_2533914 |
| Chicken polyclonal microtubule-associated protein 2 (MAP2) (1:2000) | Abcam | Cat# ab92434, RRID: AB_2138147 |
| Mouse Monoclonal Vesicular Glutamate Transporter 1 (VGLUT1) (1:500) | Millipore | Cat# MAB5502, RRID: AB_262185 |
| Rabbit monoclonal S100 beta (1:500) | Abcam | Cat# ab52642, RRID: AB_882426 |
| Goat anti-chicken IgY H&L (Alexa Fluor 647) | Abcam | Cat# ab150171, RRID: AB_2921318 |
| Donkey anti-rabbit IgG H&L (Alexa Fluor 488) | Abcam | Cat# ab150073, RRID: AB_2636877 |
| Donkey anti-rabbit IgG H&L (Alexa Fluor 568) | Abcam | Cat# ab175470, RRID: AB_2783823 |
| Donkey anti-goat IgG H&L (Alexa Fluor 488) | Abcam | Cat# ab150129, RRID: AB_2687506 |
| Goat anti-mouse IgG H&L (Alexa Fluor 594) | Abcam | Cat# ab150116, RRID: AB_2650601 |
| Donkey anti-rabbit IgG H&L (Alexa Fluor 647) | Abcam | Cat# ab150075, RRID: AB_2752244 |
| Chemicals, peptides, and recombinant proteins | ||
| Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, LDEV-free | Corning | Cat# 354230/356230 |
| Polydimethylsiloxane (PDMS) | Dow Corning | Cat# SYLGARD 184 Silicone Elastomer kit |
| Poly-L-ornithine solution | Sigma | Cat# P4957-50ML |
| Neurobasal medium | Thermo Fisher | Cat# 21103049 |
| BrainPhys neuronal medium | STEMCELL | Cat# 05790 |
| Fluo-4, AM, cell permeant | Thermo Fisher | Cat# F14201 |
| Triton X-100 | Sigma | Cat# X100-100ML |
| Normal donkey serum | Jackson ImmunoResearch | Cat# 017-000-121 |
| Calcein-AM | Yeasen | Cat# 40747-A |
| Hoechst 33342 | Yeasen | Cat# 40731ES10 |
| DAPI | Sigma | Cat# D9542 |
| Agarose, ultra-low gelling temperature, molecular biology grade | Sigma | Cat# A2576-5G |
| DMEM/F-12 | Gibco | Cat# 11330032 |
| Penicillin/streptomycin/amphotericin B, sterile solution | Leagene | Cat# CA0077 |
| Experimental models: Cell lines | ||
| DXR0109B | ATCC | Cat# ACS-1023 |
| H9 | Wicell | Cat# Wa09 |
| Software and algorithms | ||
| CALIMA | F.D.W. Radstake et al.7 | https://aethelraed.nl/calciumimaginganalyser/index.html |
| Other | ||
| Incubator | Esco | Cat# CCL-170T-8 |
| Biological safety cabinet | Esco | Cat# 2010655 |
| Orbital shaker | NEST | Cat# 105008 |
| Vibrating microtomes (vibratomes) | Leica | Leica VT 1200 S |
| Ultra-low attachment 6-well plate | Corning | Cat# 3471 |
| Sterile microcentrifuge tubes | Axgen | Cat# MCT-150-C-S |
| 12-well confocal plate | Cellvis | Cat# P12-1.5P |
| 10-cm culture dish | Biofil | Cat# TCD010100 |
| Sterile filter pipette tips | Kirgen | Cat# KG1313; KG1212; KG1111 |
| 1-Channel mechanical pipette | Eppendorf | Cat# 3120000020, 3120000011, 3120000062 3120000046 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.