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. 2023 Sep 7;67:102878. doi: 10.1016/j.redox.2023.102878

Fig. 8.

Fig. 8

SF effects on amino acids related to glutathione metabolism are altered in NRF2 KD HepG2 cells. (A) Western blot analysis of NRF2 protein in NRF2-KD HepG2 treated with 10 μM SF overnight. sVisualised protein lysates. (B) Band Intensity was quantified using Fiji, and NRF2 was normalised to beta-actin. p=0.03. (C) Cysteine extracellular levels WT DMSO vs WT SF p= 0.0002, WT DMSO vs NRF2-KD DMSO p=0.19, and NRF2-KD DMSO vs NRF2-KD SF p=0.95. (75) Glutamate intracellular levels WT DMSO vs WT SF p<0.0001, WT DMSO vs NRF2-KD DMSO p<0.0001, and NRF2-KD DMSO vs NRF2-KD SF p=0.70.(E) Serine intracellular levels, WT DMSO vs WT SF p<0.0001, WT DMSO vs NRF2-KD DMSO p=0.0004, and NRF2-KD DMSO vs NRF2-KD SF p=0.99. (F) Glycine intracellular levels WT DMSO vs WT SF p<0.0001, WT DMSO vs NRF2-KD DMSO p<0.0001, and NRF2-KD DMSO vs NRF2-KD SF p=0.95. Values are mean ± SD. Results from in vitro experiments are representative of n= 4 biological replicates.