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. 2023 Jul 13;29(18):3622–3632. doi: 10.1158/1078-0432.CCR-23-0276

Figure 3.

Figure 3. AVID200 increases GATA1 content and maturation state of the megakaryocytes in the bone marrow of patients who had an increase in platelet numbers. A, Merged confocal microscopy analysis for CD42b (red), GATA1 (green), and Hoechst (blue) of representative fields from the bone marrow of one platelet nonresponder and one platelet responder MF patient at baseline and posttreatment, as indicated. The CD42b-positive cells indicated by rectangles were also acquired in zoom mode, and the images are presented on the right. Megakaryocytes with immature or mature morphology are indicated as iMK and mMK, respectively. Plt indicates the presence of platelets. The presence or absence of green (GATA1) signal in the nucleus is indicated with a + or a –. Signals acquired in the individual red, green, and blue channels are shown in Supplementary Fig. S6A, for clarity. Similar results were obtained with two additional patients per group (see the additional images presented in Supplementary Figs. S6B and S6C). B, Frequency of CD42b-positive cells, of CD42b-positive cells with the morphology of mature megakaryocytes, of CD42b-positive cells containing some GATA1 or strongly positive for GATA1 in the bone marrow sections from nonresponder and responder patients at baseline and posttreatment, as indicated. In B, results are presented as mean (±SD) and per individual patient (each symbol represents a patient). The degree of statistical significance is indicated within the quadrant.

AVID200 increases GATA1 content and maturation state of the megakaryocytes in the bone marrow of patients who had an increase in platelet numbers. A, Merged confocal microscopy analysis for CD42b (red), GATA1 (green), and Hoechst (blue) of representative fields from the bone marrow of one platelet nonresponder and one platelet responder MF patient at baseline and posttreatment, as indicated. The CD42b-positive cells indicated by rectangles were also acquired in zoom mode, and the images are presented on the right. Megakaryocytes with immature or mature morphology are indicated as iMK and mMK, respectively. Plt indicates the presence of platelets. The presence or absence of green (GATA1) signal in the nucleus is indicated with a + or a –. Signals acquired in the individual red, green, and blue channels are shown in Supplementary Fig. S6A, for clarity. Similar results were obtained with two additional patients per group (see the additional images presented in Supplementary Figs. S6B and S6C). B, Frequency of CD42b-positive cells, of CD42b-positive cells with the morphology of mature megakaryocytes, of CD42b-positive cells containing some GATA1 or strongly positive for GATA1 in the bone marrow sections from nonresponder and responder patients at baseline and posttreatment, as indicated. In B, results are presented as mean (±SD) and per individual patient (each symbol represents a patient). The degree of statistical significance is indicated within the quadrant.