Table 1.

Sporulation conditions.

Sporulation condition	Description	Consequence
Spo8	Control	–
Modifications of Spo8 from the beginning of the growth
-CaCl2	Spo8 without CaCl2 complementation	Depletion in Ca2+ (Supplementary Figure 1A)
-MgSO4	Spo8 without MgSO4 complementation	Depletion in Mg2+ (Supplementary Figure 1B)
Modifications of Spo8 during the stationary phase
-O2	The Erlenmeyer containing the bacterial culture was sealed with a rubber stopper	Modified atmosphere (progressive decrease in the O2 availability)
-O2 + CO2	-O2 condition with the addition of 0.8% sodium bicarbonate	Modified atmosphere (progressive decrease in the O2 availability and increased CO2 concentration) and increase of pH (Supplementary Figure 1C)
H2O2	Addition of 0.005% of H2O2	Oxidative stress
Peracetic acid	Addition of 0.005% of peracetic acid	Oxidative stress and decrease of pH (Supplementary Figure 1C)
pH 5	Acidification to pH 5 by the addition of HCl	pH decrease
pH 9	Alkalinization to pH 9 by the addition of NaOH	pH increase
Benzoic acid	Addition of 0.05% C₆H₅CO₂H, a food preservative (E210)	Antibacterial properties and decrease of pH (Supplementary Figure 1C)
Nitrites	Addition of 0.05% NaNO2, a food preservative (E250)	Antibacterial properties
RBS	Addition of 0.05% RBS 25, an alkaline detergent	Detergent effect without significant impact on pH (Supplementary Figure 1C)
BAC	Addition of 0.00025% Benzalkonium chloride, a quaternary ammonium disinfectant	Antibacterial properties
UV	Ultraviolet treatment at 254 nm	Antibacterial properties

This table summarizes the different sporulation conditions used in the study. Modifications of the sporulation conditions are made from the beginning of the growth or in the early stationary phase (2 h after the transition to the stationary phase). The concentrations in peracetic acid, H₂O₂, benzoic acid, nitrites, RBS, and BAC used in these experiments correspond to the maximum concentrations of each of these agents that did not decrease the total number of CFU after 5 days of growth.