Table 2.
Influence of the sporulation conditions on surface properties of spores.
| Sporulation condition | Hydrophobicity (LogK) | Zeta potential (mV) |
|---|---|---|
| Spo8 | 0.60 ± 0.96 | -52.48 ± 5.22 |
| -CaCl2 | 2.24 ± 0.80*** | -49.48 ± 1.66 |
| -MgSO4 | 2.32 ± 0.73*** | -51.68 ± 0.83 |
| -O2 | 3.60 ± 1.03**** | -50.79 ± 1.06 |
| -O2 + CO2 | 4.30 ± 0.52**** | -50.03 ± 2.36 |
| H2O2 | 1.49 ± 0.83** | -55.98 ± 5.20 |
| Peracetic acid | 2.47 ± 1.30*** | -50.98 ± 4.75 |
| pH 5 | 2.54 ± 0.81**** | -56.52 ± 3.17* |
| pH 9 | 1.00 ± 0.44 | -49.89 ± 2.97 |
| Benzoic Acid | 1.22 ± 0.70 | -50.30 ± 1.01* |
| Nitrites | 0.29 ± 0.19 | -50.59 ± 2.07 |
| RBS | 0.21 ± 0.44 | -49.19 ± 3.69 |
| BAC | 0.85 ± 0.66 | -55.06 ± 5.26 |
| UV | 1.23 ± 0.48 | -56.37 ± 8.83 |
The surface hydrophilic/hydrophobic property and the surface charge of spores at pH 7.0 were evaluated by MATH and zetametry assays. The data presented in the table are the means with SDs. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001 for each condition versus Spo8 by Mann–Whitney or t-test.