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. 2023 Jul 4;21(10):2125–2139. doi: 10.1111/pbi.14118

Figure 3.

Figure 3

Truncated BrWAK1 by cas9‐mediated gene editing in T12–19 could weaken the resistance ability. (a) The schematic diagram of the gRNA (red arrows) location on the genome (upper panel) and conserved protein domains (lower panel) of BrWAK1. (b) Sanger‐sequencing peak map of edited sites. The mutated sites are highlighted in red. The sequences of T12–19 are on the top and those of BrWAK1 editing lines CR95 and CR80 are below it. (c) The disease symptom of BrWAK1 editing lines. Lines with non‐edited BrWAK1 were used as controls. The image in small red rectangle is magnified in the large red rectangle. (d) Images of DAB staining and callose deposition of the BrWAK1 editing lines at 24 HAI.