Figure 5.

The sequence variation in the GUB domain could confer BrWAK1 disease resistance and ability to perceive extracellular signals. (a) Schematic image of the construction of the vectors containing chimeric receptors (EGR^R‐BrWAK1 and GUB^R‐BrWAK1) and BrWAK1‐S driven by the R‐promoter (Promoter^R‐BrWAK1^S). “R” means the sequences from T12‐19 and “S” stands for those from 91 to 112. (b) The disease symptoms of the cotyledons infiltrated with chimeric vectors (EGR^R‐BrWAK1 and GUB^R‐BrWAK1) and Promoter^R‐BrWAK1^S after inoculation. The images in red rectangles are enlarged in the right panels. Cotyledons infiltrated with empty vector (pSuper1300) were used as controls. (c) The percentage of susceptible infiltrated cotyledons after inoculation. More than fifty leaves were observed after inoculation in each replicate. Values represent means ± SD (n = 3) from three biological replicates. Statistical significance between transgenic lines with empty vector (Vector) was by a t‐test: **P < 0.01. (d) The expression levels of BrWAK1 in infiltrated cotyledons after inoculation. Expression of BrWAK1 in Vector was defined as 1.0. Values represent means ± SD (n = 3) from three biological replicates. Statistical significance between Vector was by a t‐test: **P < 0.01. (e) The images of DAB staining and callose deposition of Arabidopsis seedlings transformed with BrWAK1‐S, BrWAK1‐R, EGR^R‐BrWAK1 and GUB^R‐BrWAK1 after 200 μg/mL of OG treatment.