FIGURE 2.
Vegetative growth phenotypes of ZtBCK1 and ZtCYR1 deletion strains. (a) Zymoseptoria tritici grown for 10 days on yeast peptone dextrose (YPD) at 19°C, 5‐μL droplets of a 10‐fold serial dilution starting at a concentration of 5 × 106 spores/mL. (b) Blastospores grown on YPD for 6 days, with appearance of swollen cells (arrows) in Δztbck1. Scale bar = 20 μm. (c) Growth on potato dextrose agar (PDA) at 28 and 19°C for 7 days and radial hyphal growth on water agar (WA) at 19°C for 14 days. (d) Z. tritici cell suspensions exposed 20 U/mL of lyticase enzyme from Arthrobacter luteus for 2 h at 25°C before being cultured on YPD agar for 5 days at 19°C, showing loss of viability of Δztbck1 strains at lower enzyme concentrations than the wild type IPO323.