FIGURE 3.

In planta growth and global gene transcription are impacted by deletion of ZtBCK1 and ZtCYR1. (a) Percentage of RNA sequencing reads uniquely mapped to the Zymoseptoria tritici genome across infection time points in each strain, as an indication of in planta fungal biomass. Mean values across each repeated sample are plotted along with the standard error. Statistically significant differences between percentages across time points in each strain from paired t test are indicated (*p < 0.05). (b) Change in the percentage of uniquely mapped reads between 6 and 9 days postinoculation (dpi) in each experimental repeat grouped by strain, as an indication of growth between these time points. p value from one‐way analysis of variance is given and statistically significant differences from pairwise t test comparison of mutants to IPO323 are indicated (*p < 0.05). (c) Principal component analysis (PCA) of global normalized gene expression data.