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. 2023 Jul 10;10(26):2302174. doi: 10.1002/advs.202302174

Figure 7.

Figure 7

Tissue structure/function analyses at 4 days post‐instillation. a) Representative photographs of corneal tissues excised from AB rat eyes receiving ATS (Ctrl), dexamethasone eye drop (Dex), Ce, Ce─H, and ACh+SB/Ce─H, respectively. Corneal tissue sections stained with b) H&E and c) Masson's trichrome. Scale bars are 50 µm. d) Typical TEM images and e) Young's moduli of corneal stromal tissues from different test eyes. Scale bars in d) are 0.3 µm. Values are mean ± SD (n = 10); *P < 0.05 versus all groups; # P < 0.05 versus AB, Ctrl, Dex, Ce, and Ce─H groups; ^ P < 0.05 versus Pre, AB, Ce─H, and ACh+SB/Ce‐H groups. f) α‐SMA staining of the corneas from different test eyes. Scale bars are 50 µm. g) Western blot analysis of TGF‐β and α‐SMA expression. The relative expression level of h) TGF‐β and i) α‐SMA using ImageJ software. Values are mean ± SD (n = 10); *P < 0.05 versus all groups; # P < 0.05 versus AB, Ctrl, Dex, Ce, and Ce─H groups; ^ P < 0.05 versus Pre, AB, and ACh+SB/Ce─H groups. The inclusion of the healthy (Pre) and as‐induced AB (AB) groups in all panels aims for comparative studies.