Skip to main content
. 2023 Jul 11;10(26):2302425. doi: 10.1002/advs.202302425

Figure 5.

Figure 5

ALDOA interacts with IGF2BP1 to promote mRNA translation. a) Endogenous ALDOA‐IGF2BP1 interactions in HuH‐7 cells detected by co‐IP experiments. b) Exogenous ALDOA‐IGF2BP1 interactions in 293T cells detected by co‐IP experiments. c) The interactions between HA‐tagged IGF2BP1 and endogenous ALDOA (left) or Flag‐tagged ALDOA and endogenous IGF2BP1 were detected by Co‐IP experiments. d) Immunofluorescence of Flag‐tagged ALDOA (red) colocalized with IGF2BP1 (green) in HuH‐7 (upper panels) and MHCC‐97L (lower panels) cells (n = 3). Scale bar, 50 µm. e) Schematic for IGF2BP1‐domain‐deletion mutants. f) Flag‐tagged ALDOA Co‐IP with HA‐tagged IGF2BP1 domain‐deletion mutants in 293T cells. g) OP‐Puro analysis showing that the decrease in protein synthesis induced by ALDOA knockout was reversed by IGF2BP1 overexpression in MHCC‐97L cells (n = 3). Scale bar, 50 µm. h) SUnSET analyses of MHCC‐97L (left) and SK‐Hep1 (right) cells demonstrating the changes in protein synthesis among the indicated samples. i) OP‐Puro analysis showing that the increase in protein synthesis induced by ALDOA overexpression was attenuated by IGF2BP1 knockout in SK‐Hep1 cells (n = 3). Scale bar, 50 µm.