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. 2023 Aug 31;10:rbad074. doi: 10.1093/rb/rbad074

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© The Author(s) 2023. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — KGN released from scaffolds regulates the antioxidant capacity of chondrocytes. (A, B) The ROS level of chondrocytes from various groups was evaluated by immunofluorescence staining. Scale bar = 100 μm. (C) The intracellular localization of antioxidant marker transcription factor NRF2 was evaluated by immunofluorescence staining. Scale bar = 30 μm and 100 μm. (D, E) Immunofluorescence staining of SOD2 in chondrocytes. Scale bar = 100 μm. (F) The expression of antioxidant enzyme genes (Nrf2, Sod2, and Ho-1) was determined through RT-PCR. (G, H) Western blotting was used to determine the protein level of NRF2, SOD2, and HO-1. The data are presented as mean ± SD (n = 3). *Indicates statistically significant differences where P < 0.05, ** where P < 0.01.