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. 2023 Sep 13;11(9):e007002. doi: 10.1136/jitc-2023-007002

Figure 2.

Figure 2

VIII enhances CAR-T proliferation, cytotoxicity, and cytokine secretion in vitro (A) CD4/CD8 CAR fold expansion following a 7-day co-culture with irradiated RAJI-19KO or RAJI-19WT target cell lines, ±SD/induvial data points. (B) Graphs depicting % killing of RAJI-19GFP target by NT or CAR T-cells in a 72-hour killing assay at the end of manufacture or post rechallenge where NT or CAR-T co-cultured with RAJI-19WT targets for 7 days prior. Results from all conditions were normalized to the untreated NT condition at each E:T ratio, graphs show individual data points. (C) Cytokine concentrations measured by CBA from day 3 of the 7- day co-culture with RAJI-19WT targets, graphs show individual data points. (A–C) n=6, Two-tailed Mann-Whitney U test, ns p>0.05. *p<0.05 and **p<0.01. CBA, cytokine bead array; NT, non-transduced.