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. 2023 Jul 7;19(2):302–308. doi: 10.4103/1673-5374.379038

Figure 1.

Figure 1

BrdU-immunolabeling in the cerebellar external granular of 10-day-old rats allowed to survive for 6 hours after a single injection of BrdU.

Sections representative of the vermis were treated with hydrochloric acid (3 N at 40°C for 15 minutes) (A), DNase I at 75 U/mL (B), heated sodium citrate buffer at 20 mM (C) or EcoRI (600 U/mL) combined with exonuclease III (600 U/mL) (D). BrdU-positive cells are those are those presenting a brown reaction product confined to nuclear material. Note that sections exposed to 3 N hydrochloric acid at 40°C for 15 minutes presents higher proportions of BrdU-reactive cells than those sections treated with DNase I at 75 U/mL or heated sodium citrate buffer at 20 mM. Sections incubated with EcoRI (600 U/mL) combined with exonuclease III (600 U/mL) showed the lowest proportion of labeled nuclei. Scale bar: 50 μm. Unpublished data.