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. 2023 Sep 2;101:skad298. doi: 10.1093/jas/skad298

Table 1.

Ingredient composition of experimental diets (as-fed basis)1

High E:T ratio2 Low E:T ratio2
SID Lys, % 0.82 1.22 0.82 1.22
Soybean meal 5.0 5.0 10.0 10.0
Corn 79.9 79.4 75.7 75.2
Soy protein concentrate 8.0 8.0 8.0 8.0
Soybean oil 2.5 2.5 2.5 2.5
l-Lysine 0.297 0.807 0.135 0.645
l-Arginine 0.150 0.150
dl-Methionine 0.153 0.153 0.111 0.111
l-Threonine 0.242 0.242 0.172 0.172
l-Tryptophan 0.072 0.072 0.040 0.040
l-Isoleucine 0.091 0.091
l-Valine 0.082 0.082 0.002 0.002
l-Histidine 0.053 0.053
Monocalcium phosphate 1.38 1.38 1.32 1.32
Limestone 1.32 1.32 1.32 1.32
Salt 0.40 0.40 0.40 0.40
Titanium dioxide 0.10 0.10 0.10 0.10
Vitamin/mineral premix3 0.20 0.20 0.20 0.20
Calculated nutrient content 4
E:T 0.36 0.36 0.33 0.33
Dry matter, % 87.7 89.1 88.3 88.2
Crude protein, % 15.3 15.7 16.6 17.0
ME, kcal/kg 3461 3466 3449 3454
NE, kcal/kg 2663 2667 2630 2634
Ca, % 0.77 0.77 0.77 0.77
P, % 0.38 0.38 0.38 0.38
Amino acids, % SID
Lys 0.82 1.22 0.82 1.22
Arg 0.95 0.95 0.95 0.95
His 0.40 0.40 0.40 0.40
Ile 0.59 0.59 0.59 0.59
Leu 1.20 1.20 1.32 1.32
Met + Cys 0.58 0.58 0.58 0.58
Phe + Tyr 1.02 1.02 1.17 1.17
Thr 0.67 0.67 0.67 0.67
Trp 0.19 0.19 0.19 0.19
Val 0.67 0.67 0.67 0.67

E:T, essential amino acid-nitrogen:total nitrogen ratio; ME, metabolizable energy; NE, net energy; SID, standardized ileal digestible.

1The lowest and highest Lys diets were blended in appropriate proportions to achieve diets containing the other graded levels of Lys (not shown).

2E:T ratios of 0.36 (high ratio diets) and 0.33 (low ratio diets) reflect the amount of nitrogen in the diets coming from essential amino acids (E) and from all other components (T), with the higher ratio having a larger contribution of nitrogen from essential amino acids.

3Supplied per kilogram of complete feed: vitamin A, 4,000 IU; vitamin D, 0.019 mg; vitamin E, 15 IU; vitamin B12, 0.01 mg; menadione, 1.0 mg; thiamine, 0.50 mg; riboflavin, 2.0 mg; pyridoxine, 1.0 mg; niacin, 10 mg; pantothenate, 6 mg; folic acid, 0.25 mg; biotin, 0.05 mg; Cu, 7.5 mg; Fe, 50 mg; Mg, 20 mg; I, 0.50 mg; Zn, 50 mg, and Se, 0.15 mg.

3Nutrient content of diets based on estimated nutrient contents of ingredients according to NRC (2012).