Figure 4. Mor-D mice show increased PVT→NAc^D2 and decreased BLA→NAc^D1 synaptic efficiency.

(A) Schematic of the in vivo recording of NAc D1/D2 MSNs. D1-tdTomato×D2-eGFP double transgenic mice were used to identify NAc D1/D2 MSNs. Scale bar: 100 μm. (B and C) Sample of optically evoked EPSC traces of NAc D1 MSNs and D2 MSNs from PVT or BLA inputs. The A/N ratio is shown in NAc D1 MSNs and D2 MSNs. n = 16–20 neurons from 4–5 animals per group. *P < 0.05, **P < 0.01, by 1-way ANOVA followed by Tukey’s test. (D) Flowchart of the classical high-frequency DBS treatment in bilateral NAc (130 Hz, 1 h/d for 5 days). (E) Depressive-like behaviors in mice treated with or without 130 Hz DBS. n = 16 per group Mor-nD, and n = 6 per group Mor-D. *P < 0.05, **P < 0.01, by mixed RM 2-way ANOVA followed by Šidák’s test. (F) Foot shock–induced CPP reinstatement. (G) A/N ratio in NAc of Mor-D mice. n = 18–22 neurons from 4–6 animals per group. ***P < 0.0001, by unpaired Student’s t test. Data are presented as mean ± SEM.