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. 2023 Jun 8;7(9):1097–1112. doi: 10.1038/s41551-023-01050-0

Extended Data Fig. 6. Therapeutic evaluation of IL4-aNPs.

Extended Data Fig. 6

(a) Phosphorylation of STAT6, measured by flow cytometry after 30 minutes stimulation with RPMI, IL4, or different concentrations of IL4-aNPs. The data are expressed relative to the signal elicited by bare IL4. (d) T cell polarization assay by flow cytometry, after 7 days of mixed leukocyte reaction with IL4(-aNP)-trained macrophages or control. (b) TNF production following LPS-stimulation of monocytes isolated before and 4 hours after in vivo endotoxin challenge in humans. (c) TNF and IL6 levels after ex vivo re-stimulation of human in vivo LPS-tolerized cells (left) and the same data expressed as fold changes relative to the tolerant endotoxemia samples (right).