Extended Data Fig. 5. Expansion and tumour localization of human FITC CAR T cells in NSG mice.
a In vitro cytotoxicity of FITC CAR T cells with amph-FITC+ MSTO-211H human mesothelioma cancer cells at 1:1 ET ratio. b Comparison of luciferase signal from fLuc+ CAR T cells over time in mice treated with only CD8+ 4m5.3-h28z CAR T cells or a combination of CD4+ and CD8+ CAR T cells (n = 5 animals/group). c, d Gating strategy for immunophenotyping human CAR T cells (c) and phenotypic composition of CAR T cells recovered from spleens of NSG mice 23 days post-adoptive transfer (d, n = 5 animals/group). e Quantification of CD4+ and CD8+ CAR T cells in the spleen on day 40 post-adoptive transfer (n = 5 animals/group). f Whole mouse radiance over time as a surrogate of CAR T cell expansion (n = 5 animals/group). Error bars represent standard error of the mean.