Extended Data Fig. 7. APRIL binding and NFkB signaling in BCMA wild type and mutant K562 cell lines.
(a) First histogram shows TACI expression in K562 cell line and second panel shows transgenic BCMA expression in K562 cell lines by flow cytometry. (b) Dose response curve for APRIL binding. K562 cell lines expressing wild type or mutant BCMA were incubated with Fc tagged APRIL trimer at indicated doses followed by anti-Fc APC flow antibody staining. (c) NFkB p65 ELISA was performed using nuclear extracts from K562 cell lines treated with APRIL (300 ng/ mL) for the indicated time points. (d) Western blot for phosphorylated and total ERK was performed using total cell lysates from K562 cell lines treated with APRIL (300 ng/ mL) for indicated time points.