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. 2023 Sep 15;14:5727. doi: 10.1038/s41467-023-41282-x

Fig. 4. CAR T cells reprogrammed in vivo via target cancer cells stressed by intratumoral delivery of DSF/Cu and tumor localized IR induce potent, sustained, and memory anti-solid tumor responses in multiple xenograft mouse models.

Fig. 4

a Schema of the TNBC orthotopic xenograft model (SUM159) infused with CAR T cells. b Tumor volumes of each mouse/group (n = 5 mice/group) bearing SUM159 cell line-derived tumors. c Kaplan–Meier survival curve of mice (n = 5 mice/group). d Number of infiltrated CAR T (CD3+CD45+) in tumor 5 days after CAR T infusion. e Infiltrated CAR T expressing markers associated with T-cell exhaustion (CD3+CD45+CTLA-4+ or CD3+CD45+PD-1+) in tumors 10 days after CAR T infusion of each group (n = 5 mice/group). f Representative data of early memory CAR T, defined by detection of markers CD45RA and CD62L on CD3+CD45+ B7-H3 CAR T cells found in spleen 10 days after CAR T infusion. Percentages of TSCM/TCM/TEM/TEFF are shown. g Cytokine and chemokine levels in tumors on day 5 after CAR T infusion (Tumor homogenate was pooled to yield 2 samples from 5 mice/group). h Schema of TNBC xenograft model (SUM149) infused with CAR T. i Tumor volumes of each mouse/group (n = 5 mice/group) in each group of mice bearing SUM149-derived tumors. j Percentage of CAR T cells (CD3+) in peripheral blood collected weekly from mice bearing SUM149-derived tumors (Blood was pooled to yield 3 samples from 5 mice/group). k Schema of orthotopic PDAC model (PANC-1) infused with CAR T. l Orthotopic PDAC tumor burden measured by BLI in each group treated as indicated (n = 5 mice/group). m Number of circulating CAR T (CD3+CD45+) on day 7 collected from mice bearing PDAC after T-cell injection of each group (n = 5 mice/group). n Kaplan–Meier survival curve of mice bearing PDAC in each group after tumor rechallenge (n = 5 mice/group). Statistical comparisons were performed using two-way ANOVA with Tukey’s multiple comparisons test (b, i, j), log-rank test (c, n), two-tailed unpaired t-test (d, e), and one-way ANOVA with Tukey’s multiple comparisons test (m). P-values are shown and error bars indicate mean ± SD. ns represents no significant difference. Source data are provided as a Source Data file.