REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
SARS-CoV-2 RBD primary antibody	R&D Systems	Cat#MAB105401-SP
Secondary antibody anti-mouse IgG-HRP	Jackson Immunoresearch	Cat#115-036-003; AB_2338518
Anti-mouse IgG-peroxidase rabbit antibody	Sigma-Aldrich	Cat#A9044; AB_258431
Bacterial and virus strains
SARS-CoV-2 pseudovirus	This study	N/A
Biological samples
Splenocytes isolated from immunized mice	This study	N/A
Chemicals, peptides, and recombinant proteins
ZTP (2-Amino-ATP)	TriLink Biotechnologies	Cat#N-1001
N1-Methylpseudouridine-5'-Triphosphate (m1ψTP)	TriLink Biotechnologies	Cat#N-1081
SARS-CoV-2 Spike RBD recombinant protein	Sino Biological	Cat#40592-V08B
SARS-CoV-2 Spike RBD peptide pool	JPT	Cat#PM-WCPV-S-RBD-2
Critical commercial assays
HiScribe SP6 RNA Synthesis Kit	NEB	Cat#E2070S
HiScribe T7 High Yield RNA Synthesis Kit	NEB	Cat#E2040S
Monarch RNA Clean-up Kit	NEB	Cat#T2040L
Lucigen Poly(A) Polymerase Tailing Kit	Lucigen	Cat#PAP5014H
Lipofectamine MessengerMAX	Thermo Fisher	Cat#LMRNA008
CellTiter-Glo 2.0	Promega	Cat#G9241
Mouse IFN Beta ELISA Kit	PBL	Cat#42400-1
Mouse CCL5/RANTES DuoSet ELISA kit	R&D systems	Cat#DY478-05
Human SARS-CoV-2 RBD ELISA Kit	Life Technologies	Cat#NC2008933
Mouse IL-2 ELISpot (plus) Kit	Mabtech	Cat#3441-4HPW-2
Mouse IFN-γ ELISpot (basic) Kit	Mabtech	Cat#3321-2H
Experimental models: Cell lines
HCT116	ATCC	Cat#CCL-247
HEK293T	ATCC	Cat#CRL-3216
NIH3T3	ATCC	Cat#CRL-1658
HEK293T-hACE2	BEI Resources	Cat#NR-52511
MEF	Gift from Dr. Huanyu Qiao (UIUC)	N/A
Experimental models: Organisms/strains
BALB/c mice	Jackson Laboratory	Cat#000651
Recombinant DNA
pcDNA3.1_ spike_del19	Addgene	Cat#155297
pHAGE-CMV-Luc2-IRES-ZsGreen-W	Addgene	Cat#164432
Software and algorithms
BD FACSDiva	BD Biosciences	https://www.bdbiosciences.com/en-us/products/software/instrument-software/bd-facsdiva-software
GraphPad Prism	GraphPad	https://www.graphpad.com/