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. Author manuscript; available in PMC: 2023 Sep 16.
Published in final edited form as: Cell Rep. 2023 Aug 3;42(8):112924. doi: 10.1016/j.celrep.2023.112924

Figure 7. TCF-1 directly and indirectly regulates Lta expression in PP LTi cells.

Figure 7.

(A and C) Genome Browser snapshot showing the expression of Lta and Ltb (A) and Gata3 (C) gene in Tcf7fl/fl and Tcf7fl/flRorcCre siLP LTi cells by using RNA-seq assay.

(B and D) Genomic snapshots of the Lta/Ltb loci (B) and Gata3 (D) gene regions showing the chromatin accessibility status and density of TCF-1 ChIC-seq reads in PP LTi cells from Tcf7fl/fl and Tcf7fl/flRorcCre mice. Tcf7fl/flVavCre siLP LTi cells serve as negative control group.

(E) Quantification of PPs from LTβR agonist-treated WT and Tcf7fl/flVavCre mice. Each symbol represents an individual mouse. Flow cytometric analysis of lymphotoxin expression in LTi cells from the PPs of LTβR agonist-treated WT and Tcf7fl/flVavCre mice. Quantification of LT MFI. Mean ± SD; n = 4–5 in (E); *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t test. Data are representative of two independent experiments. See also Figure S7.