| Antibodies |
|
| anti-CD117 (c-kit) BUV395 (2B8) |
BD Biosciences |
Cat# 564011; RRID: AB_2738541 |
| anti-LPAM-1 (α4β7) BV421 (DATK32) |
BD Biosciences |
Cat# 566294 |
| anti-CD335 (NKp46) PE (29A1.4) |
BD Biosciences |
Cat# 560757; RRID: AB_1727466 |
| anti-PLZF Alexa Fluor 647 (R17–809) |
BD Biosciences |
Cat# 563490; RRID: AB_2738238 |
| anti-ST2 BV711 (U29–93) |
BD Biosciences |
Cat# RRID: 745549; AB_2743075 |
| anti-GATA3 PE-CF594 (L50–823) |
BD Biosciences |
Cat# 563510; RRID: AB_2738248 |
| anti-GATA3 PE-Cy7 (L50–823) |
BD Biosciences |
Cat# 560405; RRID: AB_1645544 |
| anti-RORγt BV650 (Q31–378) |
BD Biosciences |
Cat# 564722; RRID: AB_2738915 |
| anti-RORγt PE-CF594 (Q31–378) |
BD Biosciences |
Cat# 562684; RRID: AB_2651150 |
| anti-TCF-1 PE (S33–966) |
BD Biosciences |
Cat# 564217; RRID: AB_2687845 |
| anti-CD196 (CCR6) APC (29–2L17) |
Biolegend |
Cat# 129814; RRID: AB_1877147 |
| anti-CD196 (CCR6) PE (29–2L17) |
Biolegend |
Cat# 129804; RRID: AB_1279137 |
| anti-CD127(IL-7Rα) PE-Cy7 (A7R34) |
Biolegend |
Cat# 135014; RRID: AB_1937265 |
| anti-CD127(IL-7Rα) BV421 (A7R34) |
Biolegend |
Cat# 135024; RRID: AB_11218800 |
| anti-CD45.1 BV711 (A20) |
Biolegend |
Cat# 110739; RRID: AB_2562605 |
| anti-CD45.2 Alexa Fluor 700 (104) |
Biolegend |
Cat# 109822; RRID: AB_493731 |
| anti-CD279 (PD-1) APC-Cy7 (29F.1A12) |
Biolegend |
Cat# 135224; RRID: AB_2563523 |
| anti-NK1.1 BV650(PK136) |
Biolegend |
Cat# 108735; RRID: AB_2563159 |
| anti-KLRG1 (MAFA) PE-Cy7 (2F1/KLRG1) |
Biolegend |
Cat# 138416; RRID: AB_2561736 |
| anti-CD3e PerCP-Cy5.5 (145–2C11) |
Biolegend |
Cat# 100328; RRID: AB_893318 |
| anti-CD4 BV421 (GK1.5) |
Biolegend |
Cat# 100438; RRID: AB_11203718 |
| anti-CD8a Alexa Fluor 700 (53–6.7) |
Biolegend |
Cat# 100730; RRID: AB_493703 |
| anti-B220 APC (RA3–6B2) |
Biolegend |
Cat# 103212; RRID: AB_312997 |
| anti-CD19 BV786 (6D5) |
Biolegend |
Cat# 115543; RRID: AB_11218994 |
| anti-CD3 Biotin (145–2C11) |
Biolegend |
Cat# 100304; RRID: AB_312669 |
| anti-CD19 Biotin (6D5) |
Biolegend |
Cat# 115504; RRID: AB_313639 |
| anti-Ter-119 Biotin (TER-119) |
Biolegend |
Cat# 116204; RRID: AB_313705 |
| anti-CD11b Biotin (M1/70) |
Biolegend |
Cat# 101204; RRID: AB_312787 |
| anti-CD11c Biotin (N418) |
Biolegend |
Cat# 117304; RRID: AB_313773 |
| anti-Ly-6G/Ly-6C (Gr-1) Biotin (RB6–8C5) |
Biolegend |
Cat# 108404; RRID: AB_313369 |
| anti-FcεRIα Biotin (MAR-1) |
Biolegend |
Cat# 134304; RRID: AB_1626106 |
| Streptavidin Brilliant Violet 785 (N/A) |
Biolegend |
Cat# 405249 |
| Streptavidin FITC (N/A) |
Biolegend |
Cat# 405202 |
| anti-γδ TCR Biotin (eBioGL3 (GL-3, GL3) |
Thermofisher |
Cat# 13–5711-82; RRID: AB_466668 |
| anti-CD135 (Flt3) PerCP-eFluor 710 (A2F10) |
Thermofisher |
Cat# 46–1351-82; RRID: AB_10733392 |
| anti-IgA PE (mA-6E1) |
Thermofisher |
Cat# 12–4204-82; RRID: AB_465917 |
| Fixable Viability Dye eFluor™ 506 (N/A) |
Thermofisher |
Cat# 65–0866-18 |
| LTβRIg antibody (AF.H6) |
Biogen MA, Inc. |
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
| 100 bp DNA Ladder |
Thermofisher |
Cat#15628019 |
| SYBRTM Safe DNA Gel Stain |
ThermoFisher |
Cat#S33102
|
| PlatinumTM Hot Start PCR Master Mix (2X) |
ThermoFisher |
Cat#13001012 |
| ACK Lysing Buffer |
ThermoFisher |
Cat#A1049201 |
| HBSS, calcium, magnesium |
ThermoFisher |
Cat#24020117 |
| PBS, pH 7.4 |
ThermoFisher |
Cat#10010031 |
| RPMI 1640 Medium |
ThermoFisher |
Cat#21870–076 |
| Fetal Bovine Serum |
R&D Systems |
Cat#S12450 |
| Penicillin-Streptomycin |
ThermoFisher |
Cat#15140122 |
| L-Glutamine (200 mM) |
ThermoFisher |
Cat#25030081 |
| MEM Non-Essential Amino Acids Solution (100X) |
ThermoFisher |
Cat#11140050 |
| Sodium Pyruvate |
ThermoFisher |
Cat#11360 |
| 2-Mercaptoethanol |
ThermoFisher |
Cat#21985023 |
| Phorbol 12-myristate 13-acetate |
Sigma-Aldrich |
Cat#P8139–5MG |
| Ionomycin |
Sigma-Aldrich |
Cat#407952–5MG |
| Brefeldin A Solution |
BioLegend |
Cat#420601 |
| eBioscienceTM Foxp3/Transcription Factor Staining Buffer Set |
eBioscience |
Cat#00–5523-00 |
| DNase I recombinant grade I, from bovine pancreas, expressed in Pichia pastoris |
Roche |
Cat#4536282001 |
| Liberase DH |
Roche |
Cat#5401020001 |
|
| Deposited data |
|
| Raw Data Files for RNA Sequencing |
This paper |
GEO: GSE190302
|
|
| Experimental models: Organisms/strains |
|
| Mouse: Tcf7tm1a(EUCOMM)Wtsi
|
the University of California, Davis, KOMP Repository |
www.mmrrc.org
|
| Mouse: VavCre |
JAX mice |
Stock No: 008610 |
| Mouse: RorcCre |
JAX mice |
Stock No: 022791 |
| Mouse: CreERT2 |
Taconic |
line 10471 |
| Mouse: CD45.1+
|
NIAID-Taconic repository |
line 7 |
| Mouse: CD45.1+CD45.2+
|
NIAID-Taconic repository |
line 8422 |
| Mouse: CD45.1+
Rag2−/−il2rg−/−
|
NIAID-Taconic repository |
line 8494 |
| Mouse: CD45.1+
Rag1−/− OT-II |
NIAID-Taconic repository |
line 361 |
| Mouse: Tcrb−/−d−/−
|
Dr. Amy Palin of NCI |
N/A |
| Mouse: Tcf7-YFP mice |
Dr. Bhandoola Avinash |
N/A |
|
| Software and algorithms |
|
| FlowJo v10 |
Tree Star |
https://www.flowjo.com
|
| GraphPad Prism 8 |
GraphPad |
https://www.graphpad.com
|
