Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 15;21:627. doi: 10.1186/s12967-023-04440-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — SCIC2.1 indirectly activates SIRT6 and rescues cells from metabolic stress. a Western blot analysis of SIRT6, PUMA, p21, and Bax performed in HepG2 cells treated with SCIC2.1 and Ex-527 at the indicated concentrations and time points. Heat map showing band quantification of protein expression. b SIRT6 mRNA fold expression evaluated after treatment with SCIC2.1 and Ex-527 at the indicated time points. c CETSA assay performed on HepG2 cells treated with SCIC2.1 (25 µM) and SIRT6 protein expression was analyzed. Heat map shows quantification of SIRT6 bands determined by ImageJ software. Statistical significance was calculated using the student t-test or one-way ANOVA using GraphPad Prism 9.4.0, and statistical significance is expressed as *p-value < 0.05, ^nsp-value > 0.05 vs control. Error bars represent the standard deviation (SD) of three biological replicates