Figure 5.

Impression of the effect on the balance between pro- and anti- inflammatory cytokines by Con-, HF and DTM- SCS, relative to Sham-SCS. Impression of the effect of Con-, HF- and DTM-SCS on relative RNA levels on balance of pro- and anti-inflammatory cytokines relative to Sham-SCS . The average of the log2-ΔΔCT of the anti-inflammatory cytokines was subtracted from the log2-ΔΔCT of the pro-inflammatory cytokines. A positive value indicates a shift towards more pro- and less anti-inflammatory cytokines and a negative value indicates a shift towards more anti- and less pro-inflammatory cytokines. As these experiments are limited to two pro- and two-anti-inflammatory cytokine markers, we did not perform any statistics on this analysis and is solely indicative. Con-SCS results in a balance towards more pro- and less anti-inflammatory cytokines, whilst HF- and DTM-SCS results in a balance towards less pro and more antiinflammatory cytokines.