Table 1.
Gene symbol | Gene Name | Forward and reverse primer sequence |
---|---|---|
Adgre-1 | Adhesion G protein-coupled receptor E1 | F: TTTGTGAATGCCACCTTTACC R: TGTAGCTGCACTCTGTAAGG |
Cd3d | CD3 delta subunit of T-cell receptor complex | F: GTATATGTGTAATGGGACAGAGG R: CACAGTTCTGGCACATTCG |
Eef1a1 | Eukaryotic Translation Elongation Factor 1 Alpha 1 (housekeeping) | F: TGCTGGAGCCAAGTGCTAAT R: GTGCCAATGCCGCCAATTTT |
Gapdh | Glyceraldehyde-3-phosphate dehydrogenase (Housekeeping) | F: AGACAGCCGCATCTTCTTGT R: TGATGGCAACAATGTCCACT |
Gfap | Glial fibrillary acidic protein | F: AAATCTGTGTCAGAAGGCCA R: CTCCTTAATGACCTCGCCA |
Il-1ß | interleukin 1 beta | F: CAGGAAGGCAGTGTCACTCA R: AAAGAAGGTGCTTGGGTCCT |
Il-10 | Interleukin 10 | F: TGGCCCAGAAATCAAGGAG R: GAAATCGATGACAGCGTCG |
Il-4 | Interleukin 4 | F: CAGGGTGCTTCGCAAATTTTAC R: CACCGAGAACCCCAGACTTG |
Irf-8 | Interferon regulatory factor 8 | F: TAGACCCAACAAGCTGGAG R: AGAACTGCTGCAGGTCTC |
Itgam | Integrin subunit alpha M | PrimePCR SYBR Green Assay: ITGAM (Bio-rad laboratories, Hercules (CA), USA) |
Mapk14 | Mitogen-activated protein kinase 14 | F: AGATAATGCGTCTGACGGG R: ACTGAATGTAGTTTCTTGCCTC |
P2rx4 | Purinergic receptor P2X 4 | F: CGTGGCGGACTATGTGATT R: GTGATGTTGGGGAGGATGTTC |
Tnf-α | Tumor necrosis factor α | F: CTTCTCATTCCTGCTCGTG R: TTTGGGAACTTCTCCTCCT |