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pbp2b amplicon RsaI profiles of pneumococci susceptible to penicillin and of pneumococci with low-level resistance. Lanes 1, 5, and 8 are profiles of isolates for which MICs were 0.25 μg/ml; lanes 1 and 8 are from isolates 11 and 5, respectively. Lanes 6 and 7 are profiles of isolates for which MICs were 0.12 μg/ml. Lanes 2 to 4 and 9 to 10 are profiles of penicillin-susceptible isolates (MICs, ≤0.03 μg/ml). Lane 11, Gibco-BRL 1-kb ladder.
