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. 2023 Sep 16;14:5763. doi: 10.1038/s41467-023-41488-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Faceted plots of change in median migrated cell count between alanine mutant and wild-type HD2 peptide obtained in cell migration assays (Y-axis, Δmedian migrated cell count) versus alanine mutated residue (X-axis). Chemokines used for each experiment are indicated in the strip to the right of each plot. Statistically significant differences compared to parental HD2 are shown as red dots. b Box-whisker plot showing impact of HD2 residue mutation to alanine (X-axis) upon migrated cell count (Y-axis) for all chemokines studied. All experiments were performed as three biological replicates, and individual data points are indicated, and coloured by the chemokine used. The box-whisker plot shows the median as centre, 25th and 75th percentile as bounds, and 1.5*interquartile range as whiskers. Statistically significant differences (compared to control, coloured blue), using Dunnett’s test with correction for multiple comparisons, are indicated by asterisks: ****p ≤ 0.0001, ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05, n = 15 observations per group (three biological replicates for each chemokine). Exact p-values are: C13A 1.62e−05; L11A 2.86e−02; P10A 2.79e−06; Y14A 4.17e−02. c Correlation of phage binding and inhibitory potency for alanine mutant HD2 peptides. Scatterplot of Δmedian migrated cell count (Y-axis, obtained in cell migration assays) versus Δlog2E (X-axis, obtained in phage-display experiments) for peptide:chemokine pairs. Individual data points indicate peptide chemokine pairs for which both cell migration and Δlog2E were available. Linear regression plot with 95% confidence interval, Spearman correlation coefficient (R) and statistical significance (p) are shown. Source data are provided as a Source Data file.