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. 2023 Sep 16;10:631. doi: 10.1038/s41597-023-02546-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The workflow of generating haplotype-resolved and telomere-to-telomere reference genome for M. acuminata. The unphased reference genome was constructed following the workflow on the left yellow panel. Then a haplotype-resolved and telomere-to-telomere reference genome was produced according to the scheme on the right blue panel. Green boxes represent raw sequencing data; white boxes represent the tools used in this pipeline; pink boxes represent intermediate data; blue boxes represent post-analysis process.